| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1221 | Bacillus smithii | GCAAAGTTTACCGCACGGC | AGGGCATCCACCGTTTCAA | 60.73 | 59.54 | 291 | 63 |
100.00%
|
100.00%
|
| Primer 1222 | Bacillus smithii | TTGCAAAGTTTACCGCACGG | AGGGCATCCACCGTTTCA | 59.97 | 58.83 | 293 | 63 |
100.00%
|
100.00%
|
| Primer 1223 | Bacillus smithii | TGCAGGTGCGCTTCTTCA | GCATGCCTGGCGAAACAA | 59.89 | 59.35 | 160 | 63 |
100.00%
|
100.00%
|
| Primer 1224 | Bacillus smithii | GGAGTCGCCTGGTTTCCAA | TCCCATCGCAATGCCCAT | 59.93 | 59.40 | 292 | 63 |
100.00%
|
100.00%
|
| Primer 1225 | Bacillus smithii | ACCTTTCCCCTGTTGTCGG | TGCAAGGCTTCGTCCACT | 59.55 | 59.17 | 289 | 63 |
100.00%
|
100.00%
|
| Primer 1226 | Bacillus smithii | TTATCGTGCCAGACAGCGG | TTGCTGCATTCCACCGGA | 60.15 | 59.57 | 248 | 63 |
100.00%
|
100.00%
|
| Primer 1227 | Bacillus smithii | TGCCAACGGAATCACCCAA | TGCTGCGCACAATGAACG | 59.85 | 59.75 | 171 | 63 |
100.00%
|
100.00%
|
| Primer 1228 | Bacillus smithii | TCGCGATTTTGCCAAGGC | TCGGAAATGACCTCACCGG | 59.43 | 59.41 | 273 | 63 |
100.00%
|
100.00%
|
| Primer 1229 | Bacillus smithii | AACGGTTGACAGGACGCA | TTTCCTGCGTGCCGTCAA | 59.50 | 60.20 | 237 | 63 |
100.00%
|
100.00%
|
| Primer 1230 | Bacillus smithii | AACGGGTGAAACGGGTTGT | TGCCAAGTTGATGCGCAA | 60.08 | 58.56 | 198 | 63 |
100.00%
|
100.00%
|